Indocyanine green fluorescence-navigated laparoscopic metastasectomy for peritoneal metastasis of hepatocellular carcinoma: a case report

Indocyanine green (ICG) is used not only for evaluation of liver function but also for surgical navigations as it emits fluorescence upon exposure to near-infrared illumination [1]. Selective uptake and retention of ICG by hepatocellular carcinoma (HCC) tumors have recently been revealed, and ICG fluorescence imaging has begun to be applied as an extremely sensitive intraoperative tool for detecting foci of HCC [2, 3]. Recent reports have demonstrated that ICG can selectively accumulate in primary HCC and extrahepatic metastases of HCC; additionally, ICG fluorescence imaging presented clear boundaries between tumors and normal tissue [2–5]. Here, we report, to the best of our knowledge, the first case of laparoscopic resection of peritoneal metastasis using ICG fluorescence imaging.

To the best of our knowledge, our case is the first to report the use of ICG fluorescence-navigated laparoscopic metastasectomy in a patient with peritoneal metastasis of HCC. This method is advantageous in facilitating the intraoperative identification of metastatic lesions, particularly during laparoscopic surgery in which the tumor is not palpable.

The survival benefit of removing extrahepatic metastatic lesions of HCC is a clinical question that has not yet been clarified. Long-term survivors in selected cases have been reported in previous literatures [6, 7]. In our institution, the surgical indications for extrahepatic metastases of HCC included all countable lesions that are macroscopically resectable, with intrahepatic lesions that are resectable or controllable by other treatment modalities. In this case, there were no other metastases, and resection of the solitary metastasis was performed.

Recent reports have demonstrated that ICG can selectively accumulate not only in primary HCC but also extrahepatic metastases [2–5]. ICG is selectively accumulated by the hepatocytes and excreted in the bile via an active transport system. However, owing to canalicular transporter anomalies, ICG is captured by HCC cells but cannot be excreted correctly in the biliary canalicules, and thus, ICG accumulates in HCC cells [8]. Ishizawa et al. reported that the fluorescent patterns were classified into three types: total, partial, and rim fluorescent types; these were correlated with tumor differentiation [3]. ICG can accumulate in peritoneal metastases because of their histological similarity to the original HCC. Therefore, ICG fluorescence imaging confirmed the lesion as a peritoneal metastasis. A clinical issue pertaining to this method is the optimal interval between ICG administration and surgery. For detecting an intrahepatic lesion, an interval of at least 2 days is recommended; however, to detect extrahepatic HCC metastasis, the contrast against the liver parenchyma is not essential; the recommended interval is 1–5 days.

ICG-NIF imaging system is advantageous in laparoscopic surgery in which the tumor is not palpable. Laparoscopic surgery is becoming increasingly widespread due to technological developments and improvements in endoscopic technique. However, one disadvantage of laparoscopic surgery is that it is difficult to confirm the tumor and resection margins, owing to the inability to palpate the tumor. Intraoperative ultrasonography is generally useful for detecting liver tumors during laparoscopic hepatectomy. However, regarding small disseminated tumors on the peritoneum, ultrasonography is not useful. The benefit of this ICG fluorescence method is that small metastatic lesions can be easily identified during laparoscopic surgery. Reportedly, IGC fluorescence imaging system can detect 3-mm HCC metastatic lesions [2, 4]. The sensitivity and positive predictive value of intraoperative ICG fluorescent imaging were reported to be 92% and 100%, respectively [4]. However, a limitation of this method is that it cannot detect fluorescence when a lesion is deeply located in a tissue. Thus, tumors at a depth of > 5–10 mm from the surface could not be identified [2–4]. In this case, the tumor location could be visualized by the ICG-NIF imaging system, and we could detect and resect the metastatic tumor on the peritoneum. Without the ICG-NIF imaging system, the peritoneal tumor could not be detected because the tumor was not visible in the surrounding hard adhesion and adipose tissue.

In conclusion, we presented a case of laparoscopic resection of peritoneal metastasis using ICG fluorescence imaging. This method is useful for the detection and safe resection of peritoneal tumors during laparoscopic surgery.