- Case report
- Open Access
A case of napsin A-positive metastatic lung cancer originating from the colon
Surgical Case Reports volume 3, Article number: 114 (2017)
We report a case of napsin A-positive metastatic lung cancer originating from the colon. No cases of napsin A-positive metastatic lung tumors originating from colorectal cancer have been reported previously.
Computed tomography identified a small lung nodule in a 70-year-old male patient, 18 months after resection for rectal cancer. The size of the lung tumor increased from 1.8 to 2.1 cm in 6 months and metastasis from the rectal cancer was suspected. Resection of the lung tumor was performed, and the histological features of the lung tumor revealed findings typical of colorectal adenocarcinoma and resembled those of the original rectal cancer. Furthermore, the metastasis stained positive for napsin A and thyroid transcription factor-1 (TTF-1) on immunohistochemical evaluation, and immunohistochemical analysis identified the same results in the rectal specimen.
These findings led us to believe that this was a rare case of napsin A-positive metastatic lung cancer originating in the colon. The patient was treated with chemotherapy for recurrent rectal cancer, and no other metastases were found after the lung resection. This is the first report of napsin A-positive colorectal cancer metastasizing to the lung.
Napsin A and thyroid transcription factor-1 (TTF-1) are known to be useful immunohistochemical markers to distinguish primary lung cancers from metastatic lung tumors. These markers are usually positive in primary lung adenocarcinomas but not metastatic adenocarcinomas in other organs. Napsin A, especially, is rarely expressed in colon adenocarcinomas and has high specificity in lung adenocarcinoma. No cases of Napsin A-positive metastatic lung tumors originating from colorectal cancer have been reported previously.
We report a patient with a metastatic lung tumor originating from rectal cancer that was positive for both napsin A and TTF-1 expression.
A 70-year-old man underwent colorectal resection for rectal cancer (well-differentiated tubular adenocarcinoma, pT3a N0, stage IIA). At this time, no pulmonary lesion was detected on computed tomography (CT) (Fig. 1a). Eighteen months later, a well-defined solid nodule appeared in the upper lobe of the right lung on CT (Fig. 1b). The diameter of the nodule was 0.8 cm. After a further 6 months, the diameter increased up to 2.1 cm (Fig. 1c). Positron emission tomography revealed high uptake in the nodule. Serum levels of carcinoembryonic antigen and CA19-9 were within the normal range, and no recurrence of the rectal cancer was found.
Based on radiological findings and the speed of growth, the lung nodule was clinically diagnosed as a metastatic tumor originating from the rectal cancer. Thoracoscopic wedge resection of the right upper lung lobe was subsequently performed. A frozen section of the resected tumor was examined perioperatively. The intraoperative pathological diagnosis was metastatic adenocarcinoma, and the surgery was completed without additional resection. The postoperative course was uneventful, and the patient was discharged on the fourth postoperative day.
Further pathological examination of the surgical specimens including the previously resected rectal cancer was performed. The histological features of the lung tumor revealed findings typical of colorectal adenocarcinoma (Fig. 2a1) and resembled those of the original rectal cancer (Fig. 2b1). Furthermore, immunohistochemical study of the lung tumor was performed with multiplex antibody reagent (Pathology Institute corporation, Toyama, Japan) containing napsin A (clone: TMU-Ad02) and TTF-1 (clone: SPT24). The study showed that both markers were positive. The specimens were reexamined with polyclonal napsin A antibodies (Nichirei Biosciences Inc., Tokyo, Japan) and the rabbit monoclonal TTF-1 antibody (clone: SP141, Roche) in separate experiments. In addition, we obtained an immunohistochemical stain from a third-party laboratory (Kyodo Byori Inc., Kobe, Japan). They used two monoclonal antibodies; napsin A (clone: IP64, Leica Biosystems Newcastle Ltd.) (Fig. 2a2, b2); TTF-1 (clone: SPT24, Novocastra) (Fig. 2a3, b3). All results were positive for both markers. Thus, we finally diagnosed the lung nodule as a metastasis from the napsin A-positive rectal cancer.
The patient then received eight cycles of combined chemotherapy with oxaliplatin and capecitabine. The patient is alive without any recurrence 34 months after the first surgery for primary rectal cancer.
To our knowledge, this is the first report of a napsin A-positive metastatic lung tumor originating from colon cancer. Napsin A is generally expressed in lamellar bodies of type II pneumocytes . Therefore, napsin A is highly expressed in primary lung adenocarcinomas, but not metastatic lung adenocarcinomas . The specificity of napsin A expression is reliable especially as compared with colon adenocarcinomas. Renal cell carcinomas and a few other adenocarcinomas are known to be occasionally positive for napsin A; however, no positive colon adenocarcinoma cases have been reported .
It has been reported that polyclonal napsin A antibody sometimes reacts nonspecifically to non-pulmonary adenocarcinomas . In our case, immunohistochemical examination was performed with various antibodies: cocktailed, polyclonal, and monoclonal. Reexamination was performed at a third-party laboratory, and all the results were positive in the both the lung tumor and rectal cancer.
The diagnosis in our case was metastatic lung cancer originating from primary rectal cancer. The histological features of the lung tumor were typical of a primary rectal adenocarcinoma. The radiological features of the lung tumor were also typical of a metastatic lung tumor. Considering the clinical course of the patient, it is irrational to believe that a primary lung cancer appeared 18 months after resection of a metastatic rectal cancer.
The lung cancer and the rectal cancer could not have been independent of each other. The results of a hematoxylin and eosin stain were similar in both lesions, as was the protein expression pattern; as such, both adenocarcinomas likely share a common origin. No other malignant lesions have been found 21 months after the last surgery.
In this patient, the colon adenocarcinoma was positive for napsin A. Some thyroid carcinomas have also been reported to be positive for napsin A, which were all of the papillary type with tall cell morphology . Our patient’s rectal cancer also exhibited these features. The reasons for positive staining of napsin A in our case remain unknown. The rectal cancer may have produced some proteins that cross-react to napsin A  or there may be issues with the sensitivity of the antibodies used for immunohistochemical analysis.
One case of TTF-1-positive metastatic lung cancer originating from colon cancer has already been reported . The expression of TTF-1 in primary lung adenocarcinomas is high but lower than napsin A. TTF-1 expression is rarely positive in cancers of the colon, uterus, ovaries, and so on .
We report a case of napsin A- and TTF-1-positive metastatic lung cancer originating from rectal cancer. Clinicians and pathologists should be aware of the existence of false-positive cases like this and be extra vigilant in similar cases in the future.
Thyroid transcription factor-1
Ueno T, Linder S, Elmberger G. Aspartic proteinase napsin is a useful marker for diagnosis of primary lung adenocarcinoma. Br. J. Cancer. 2003;88:1229–33.
Siddiqui M. TTF-1 and napsin a double staining in diagnosing lung adenocarcinoma. J Cytol Histol. 2012;3:e103.
Bishop JA, Shrma R, Illei B. Napsin A and thyroid transcription factor-1 expression in carcinomas of the lung, breast, pancreas, colon, kidney, thyroid, and malignant mesothelioma. Hum Pathol. 2010;41:20–5.
Rekhtman N, Kazi S. Nonspecific reactivity of polyclonal napsin A antibody in mucinous adenocarcinomas of various sites: a word of caution. Arch Patol Lab Med. 2015;139:434–6.
Kadivar M, Boozari B. Applications and limitations of immunohistochemical expression of “Napsin-A” in distinguishing lung adenocarcinoma from adenocarcinomas of other organs. Appl Immunohistochem Mol Morphol. 2013;21:191–5.
Belalcazara A, Krishnamurti U, Shaib W. TTF-1 expression in rectal adenocarcinoma: a case report and review of the literature. Case Rep Oncol. 2016;9:747–51.
Jagirdar J. Application of immunohistochemistry to the diagnosis of primary and metastatic carcinoma to the lung. Arch Pathol Lab Med. 2008;132:384–96.
We would like to thank Editage (www.editage.jp) for English language editing.
The authors declare no financial or any other type of support.
Ethics approval and consent to participate
The ethics committee of Saitama Hospital approved this study (R2017-07).
Consent for publication
The patient has provided permission to publish these features of the cases, and the identity of the patient has been protected.
The authors declare that they have no competing interests.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
About this article
Cite this article
Ito, T., Nakanishi, K. & Goto, H. A case of napsin A-positive metastatic lung cancer originating from the colon. surg case rep 3, 114 (2017). https://doi.org/10.1186/s40792-017-0389-9
- Immunochemical stain
- Colon cancer
- Lung cancer