Fibrolamellar hepatocellular carcinoma: a case report and gene analysis

Surgical Case Reports

Table 1 Comparison of gene expression between FL-HCC and young HCC

Gene Symbol	Gene Expression		P-value	Fold Change
	FL-HCC	HCC <40 y.o.
	(n=5)	(n=29)
VCAN	8.817148	1.616	< 0.001	7.2011
CCDC80	10.88519	4.140918	0.001	6.7442
NTS	6.840951	1.106541	< 0.001	5.7344
PCSK1	11.88166	6.896194	< 0.001	4.9854
AKAP12	6.534875	1.747636	< 0.001	4.7872
TF	6.011128	1.56449	< 0.001	4.4466
COL1A2	7.14167	2.956288	0.0014	4.1853
COL1A1	5.985437	2.101835	< 0.001	3.8836
COL3A1	7.628198	3.775831	0.001	3.8523
CRP	6.269426	2.442686	< 0.001	3.8267
SLC16A14	7.643811	3.823263	< 0.001	3.8205
DCN	8.26088	4.526932	< 0.001	3.7339
EPHX1	8.34207	4.626477	0.008	3.7155
PLA2G2A	7.922695	4.240189	0.0067	3.6825
CTH	7.742455	4.069396	0.0014	3.673
TMEM45A	8.165952	4.50216	0.0217	3.6637
LUM	6.046244	2.411356	0.0029	3.6348
OAT	5.642841	2.057272	0.001	3.5855
F2	8.380275	4.797066	< 0.001	3.5832
RARRES2	10.3543	6.812451	0.0159	3.5418
SERPIND1	4.699167	1.193037	< 0.001	3.5061
KRT7	5.719496	7.844187	0.0018	− 2.1246
APOA2	8.785837	10.92171	0.0252	− 2.1358
MUC13	4.904502	7.130208	0.0436	− 2.2257
TAT	4.591301	6.93159	0.0382	− 2.3402
AHSG	4.938774	7.312314	0.0187	− 2.3735
HPD	6.358531	8.736732	0.0217	− 2.3782
SCD	6.214376	8.63604	0.0014	− 2.4216
SDS	7.935027	10.36979	0.0382	− 2.4347
SERPINC1	6.672331	9.16285	< 0.001	− 2.4905
APOE	6.04407	8.785136	< 0.001	− 2.741
TTR	4.848222	7.63215	0.0023	− 2.7839
GSTA1	4.605411	7.427156	0.0252	− 2.8217
APOA1	8.581028	11.9013	0.0029	− 3.3202
FABP1	4.335924	8.726212	0.0115	− 4.3902

FL-HCC (n = 5: primary and lymphatic metastasis lesion in our case and previous 3 cases)
The gene names was referred the newest symbol of HGNC (HUGO Gene Nomenclature Committee)
The gene expression level was used by taking the FPKM value of mRNA as log2(x + 1)
P value was evaluated by Wilcoxon rank sum test. Fold Change is the expression ratio of FL-HCC to younger HCC