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Fig. 1 | Surgical Case Reports

Fig. 1

From: Long-term outcome of a dilated cardiomyopathy patient after mitral valve surgery combined with tissue-engineered myoblast sheets—report of a case

Fig. 1

(1-1) The autologous myoblast sheets were round and about 5 cm in diameter. The purity of the myoblasts in the sheets was over 90%. (1-2) HE staining revealed a large number of cells connected to each other, with abundant extracellular matrix, in a cell sheet that was about 100-μm-thick. (1-3) Almost all of the cells in the cell sheet showed positive immunostaining for desmin in the cytosol. Green, desmin; blue, DAPI. (1-4) Almost all of the cells in the cell sheet showed positive immunostaining for actin in the cytosol. Red, actin; blue, DAPI. (1-5) Stacked autologous myoblast sheets were implanted onto six areas of the epicardium on the lateral and anterior LV wall through the fifth intercostal space

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