Fig. 1

Representative hematoxylin–eosin-stained images and CD3⁺ immunohistochemistry results in primary gallbladder cancer specimen. a Specimen with tumor-infiltrating lymphocytes. Right: × 200; left (insert): × 50. b Lymphocytes infiltrate tumor stroma. Brown chromogen: CD3⁺ T cells. Right: × 200; left (insert): × 50