Skip to main content
Surgical Case Reports
Fig. 2 | Surgical Case Reports

Fig. 2

From: Hereditary breast cancer associated with Cowden syndrome-related PTEN mutation with Lhermitte-Duclos disease

Fig. 2

Analysis of PTEN gene mutations. Genomic DNAs for germ line mutation analyses were obtained from the buffy coat and resected tumor. Polymerase chain reaction products were sequenced directly using an automated DNA-sequencing system (Model 3130; Applied Biosystems, Foster City, CA). PTEN sequencing demonstrated that a germ line mutation c. 288 insertion A was found in exon 8, which caused a frameshift mutation (p.V290fs*8). A reverse primer was used in this analysis

Back to article page